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Flow cytometric analysis of hUCESC. Representative figure of hUCESC labeled with FITC- and PE- antibodies and analyzed by flow cytometry. Histograms show the expression of surface antigens.

Journal: Biomolecules

Article Title: Secretome from Uterine Cervical Mesenchymal Stem Cells as a Protector of Neuronal Cells Against Oxidative Stress and Inflammation

doi: 10.3390/biom15101402

Figure Lengend Snippet: Flow cytometric analysis of hUCESC. Representative figure of hUCESC labeled with FITC- and PE- antibodies and analyzed by flow cytometry. Histograms show the expression of surface antigens.

Article Snippet: In order to characterize the isolated hUCESC, the cells were stained with a panel of specific monoclonal antibodies for mesenchymal stem cells: CD31-PE, CD34-PE, CD44-PE, CD45-FITC, CD73-PE (Becton Dickinson, Biosciences Pharmingen, San Diego, CA, USA), CD90-FITC, and CD105-FITC (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Labeling, Flow Cytometry, Expressing

ECM coating using the layer-by-layer (LbL) technique was deposited on the cell surface. (A) Gelatin-RBITC/HyA-FITC-coated hMSCs via FACS analysis were shifted to the positive region (Gelatin-RBITC in red fluorescence and HyA–FITC in green fluorescence). (B) Mean fluorescence intensity (MFI) of the ECM coating layer on hMSC was changed with deposition of increasing numbers of layers based on FACS analysis ( n = 4).

Journal: Biomaterials Research

Article Title: External-Force-Offset Effects of ECM Coating Layers on hMSCs Subjected to External Physical Force

doi: 10.34133/bmr.0265

Figure Lengend Snippet: ECM coating using the layer-by-layer (LbL) technique was deposited on the cell surface. (A) Gelatin-RBITC/HyA-FITC-coated hMSCs via FACS analysis were shifted to the positive region (Gelatin-RBITC in red fluorescence and HyA–FITC in green fluorescence). (B) Mean fluorescence intensity (MFI) of the ECM coating layer on hMSC was changed with deposition of increasing numbers of layers based on FACS analysis ( n = 4).

Article Snippet: 153037, Mouse anti Human CD90: FITC Bio-Rad Formerly AbD Serotec), and CD105 (endoglin, Lot.

Techniques: Fluorescence

Surface morphological changes in bare hMSCs and ECM-hMSCs. (A) Confocal laser scanning microscopy (CLSM) images of Gelatin-RBITC (red)/HyA-FITC (green)-coated hMSCs. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) in blue (magnification: ×400, scale bar: 20 μm). (B) The cell diameter of bare hMSCs and ECM-hMSCs was calculated using ImageJ based on the images of CLSM ( n = 50). (C) Scanning electron microscope (SEM) image depicting the cell surface morphology between the bare hMSCs and ECM-hMSCs independent experiments (inner image—magnification: ×5.0 k, scale bar: 10 μm; outer image—magnification: ×10.0 k, scale bar: 5 μm).

Journal: Biomaterials Research

Article Title: External-Force-Offset Effects of ECM Coating Layers on hMSCs Subjected to External Physical Force

doi: 10.34133/bmr.0265

Figure Lengend Snippet: Surface morphological changes in bare hMSCs and ECM-hMSCs. (A) Confocal laser scanning microscopy (CLSM) images of Gelatin-RBITC (red)/HyA-FITC (green)-coated hMSCs. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) in blue (magnification: ×400, scale bar: 20 μm). (B) The cell diameter of bare hMSCs and ECM-hMSCs was calculated using ImageJ based on the images of CLSM ( n = 50). (C) Scanning electron microscope (SEM) image depicting the cell surface morphology between the bare hMSCs and ECM-hMSCs independent experiments (inner image—magnification: ×5.0 k, scale bar: 10 μm; outer image—magnification: ×10.0 k, scale bar: 5 μm).

Article Snippet: 153037, Mouse anti Human CD90: FITC Bio-Rad Formerly AbD Serotec), and CD105 (endoglin, Lot.

Techniques: Confocal Laser Scanning Microscopy, Staining, Microscopy